B6916

Sigma

 

Bradford Reagent

for 1-1,400 μg/ml protein

Synonym:Coomassie® dye binding protein assay, Protein dye reagent

Description

Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.
ApplicationThis protein assay is based on complexing of proteins with Brilliant Blue G. The protein sample is mixed with the reagent and then read at 595 nm after a short incubation at room temperature.
Features and Benefits• The reagent is ready to use. No mixing or dilution required.
• Color development is rapid. Only a five minute incubation and then the sample is read a 595 nm.
• Reducing sugars and reducing substances along with thiols do not interfere with this reagent.
• Reagent is suitable for micro (1-10 μg/ml) and standard (50-1400 μg/ml) assays.
• Can be used in microwell plate assays.
• Inexpensive assay.
PackagingData sheet included with each bottle.
Legal InformationCoomassie is a registered trademark of Imperial Chemical Industries Ltd.

Properties

storage temp.2-8°C

Safety

Hazard CodesC
Risk Statements20/21/22-34-68/20/21/22
Safety Statements26-36/37/39-45
RIDADRUN 1805 8/PG 3
WGK Germany1

Related Products

Related productTP0100, Total Protein Kit, Micro
ApplicationFP0010, FluoroProfile® Protein Quantification Kit

References

referenceBradford, M., A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of dye-binding. Anal. Biochem. 72, 248-254, (1976) Abstract
 Compton, S.J., and Jones, C.G., Mechanism of dye response and interference in the Bradford protein assay. Anal. Biochem. 151, 369-374, (1985) Abstract
 Tal, M., et al., Why does Coomassie Brilliant Blue interact differently with different proteins? A partial answer. J. Biol. Chem. 260, 9976, (1985) Abstract
 Splittgerber, A.G., Sohl, J., Nonlinearity in protein assays by the Coomassie blue dye-binding method. Anal. Biochem. 179, 198, (1989) Abstract
 Rubin, R.W., and Warren, R.W., Quantitation of microgram amounts of protein in SDS-mercaptoethanol-tris electrophoresis sample buffer. Anal. Biochem. 83, 773-777, (1977) Abstract
 Friedenauer, D., and Berlet, H.H., Sensitivity and variability of the Bradford protein assay in the presence of detergents. Anal. Biochem. 178, 263-268, (1989) Abstract